mouse flt3l elisa kit Search Results


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The Mouse Flt-3L ELISA research-use-only kit is an enzyme-linked immunosorbent assay designed for the quantitation and detection of Flt-3L in samples (see sample types indicated) using a microplate reader. The assay will recognize both natural
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93
R&D Systems quantikine elisa kits
a , Total cell numbers in BM, thymus, spleen and lymph nodes from WT ( n = 12), NOD1 −/− ( n = 10), ΔCARD NOD1 ( n = 10), NOD2 −/− ( n = 6) or MyD88 −/− ( n = 6) mice. b , Absolute numbers of T and B lymphocytes and NK cells in spleen from WT, NOD1 −/− or ΔCARD NOD1 ( n = 10) mice. c , Level of IL-7, <t>Flt3L,</t> thrombopoietin (ThPO) or SCF measured in sera of individual WT, NOD1 −/− and ΔCARD NOD1 ( n = 13) mice by <t>ELISA.</t> d , Total numbers of LT-HSCs (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 neg ), short-term HSCs (ST-HSCs) (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 + ), CLPs (Lin neg IL-7Rα + Flt3 + Sca1 inter c-kit inter ) and common myeloid progenitors (CMPs) (Lin neg IL-7Rα neg Sca-1 neg c-kit + CD16/32 neg CD34 + ) in the BM of WT, NOD1 −/− or ΔCARD NOD1 ( n = 16) mice. e , Frequency of donor cells in BM, thymus and spleen 8 weeks after reconstitution of irradiated H-2K/D d/b WT recipient mice ( n = 5) with a combination of equal numbers of congenically marked sort-purified HSCs (Lin neg IL-7Rα neg c-kit + Sca-1 + Flt3 neg ) from H-2K/D b/b WT (CD45.1/2 + ), NOD1 −/− (CD45.2 + ) and ΔCARD NOD1 (CD45.1 + ) animals as indicated on the diagram. a – d , Each symbol represents the value for an individual mouse. The bars indicate mean ± s.e.m. value for each group pooled from three experiments ( a – c ) and from one representative out of two independent experiments performed ( d ). Significance was determined by two-sided Mann–Whitney test in a , b and e and one-way ANOVA (Tukey’s multiple comparisons) test in c and d and are indicated by asterisks: * P < 0.05, ** P < 0.01, *** P < 0.001.
Quantikine Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a , Total cell numbers in BM, thymus, spleen and lymph nodes from WT ( n = 12), NOD1 −/− ( n = 10), ΔCARD NOD1 ( n = 10), NOD2 −/− ( n = 6) or MyD88 −/− ( n = 6) mice. b , Absolute numbers of T and B lymphocytes and NK cells in spleen from WT, NOD1 −/− or ΔCARD NOD1 ( n = 10) mice. c , Level of IL-7, <t>Flt3L,</t> thrombopoietin (ThPO) or SCF measured in sera of individual WT, NOD1 −/− and ΔCARD NOD1 ( n = 13) mice by <t>ELISA.</t> d , Total numbers of LT-HSCs (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 neg ), short-term HSCs (ST-HSCs) (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 + ), CLPs (Lin neg IL-7Rα + Flt3 + Sca1 inter c-kit inter ) and common myeloid progenitors (CMPs) (Lin neg IL-7Rα neg Sca-1 neg c-kit + CD16/32 neg CD34 + ) in the BM of WT, NOD1 −/− or ΔCARD NOD1 ( n = 16) mice. e , Frequency of donor cells in BM, thymus and spleen 8 weeks after reconstitution of irradiated H-2K/D d/b WT recipient mice ( n = 5) with a combination of equal numbers of congenically marked sort-purified HSCs (Lin neg IL-7Rα neg c-kit + Sca-1 + Flt3 neg ) from H-2K/D b/b WT (CD45.1/2 + ), NOD1 −/− (CD45.2 + ) and ΔCARD NOD1 (CD45.1 + ) animals as indicated on the diagram. a – d , Each symbol represents the value for an individual mouse. The bars indicate mean ± s.e.m. value for each group pooled from three experiments ( a – c ) and from one representative out of two independent experiments performed ( d ). Significance was determined by two-sided Mann–Whitney test in a , b and e and one-way ANOVA (Tukey’s multiple comparisons) test in c and d and are indicated by asterisks: * P < 0.05, ** P < 0.01, *** P < 0.001.
E El M0030 Mouse Crp Elisa Kit Beyotime, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems cytokine flt3l elisa
a , Total cell numbers in BM, thymus, spleen and lymph nodes from WT ( n = 12), NOD1 −/− ( n = 10), ΔCARD NOD1 ( n = 10), NOD2 −/− ( n = 6) or MyD88 −/− ( n = 6) mice. b , Absolute numbers of T and B lymphocytes and NK cells in spleen from WT, NOD1 −/− or ΔCARD NOD1 ( n = 10) mice. c , Level of IL-7, <t>Flt3L,</t> thrombopoietin (ThPO) or SCF measured in sera of individual WT, NOD1 −/− and ΔCARD NOD1 ( n = 13) mice by <t>ELISA.</t> d , Total numbers of LT-HSCs (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 neg ), short-term HSCs (ST-HSCs) (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 + ), CLPs (Lin neg IL-7Rα + Flt3 + Sca1 inter c-kit inter ) and common myeloid progenitors (CMPs) (Lin neg IL-7Rα neg Sca-1 neg c-kit + CD16/32 neg CD34 + ) in the BM of WT, NOD1 −/− or ΔCARD NOD1 ( n = 16) mice. e , Frequency of donor cells in BM, thymus and spleen 8 weeks after reconstitution of irradiated H-2K/D d/b WT recipient mice ( n = 5) with a combination of equal numbers of congenically marked sort-purified HSCs (Lin neg IL-7Rα neg c-kit + Sca-1 + Flt3 neg ) from H-2K/D b/b WT (CD45.1/2 + ), NOD1 −/− (CD45.2 + ) and ΔCARD NOD1 (CD45.1 + ) animals as indicated on the diagram. a – d , Each symbol represents the value for an individual mouse. The bars indicate mean ± s.e.m. value for each group pooled from three experiments ( a – c ) and from one representative out of two independent experiments performed ( d ). Significance was determined by two-sided Mann–Whitney test in a , b and e and one-way ANOVA (Tukey’s multiple comparisons) test in c and d and are indicated by asterisks: * P < 0.05, ** P < 0.01, *** P < 0.001.
Cytokine Flt3l Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Rockland Immunochemicals stem cell factor
a , Total cell numbers in BM, thymus, spleen and lymph nodes from WT ( n = 12), NOD1 −/− ( n = 10), ΔCARD NOD1 ( n = 10), NOD2 −/− ( n = 6) or MyD88 −/− ( n = 6) mice. b , Absolute numbers of T and B lymphocytes and NK cells in spleen from WT, NOD1 −/− or ΔCARD NOD1 ( n = 10) mice. c , Level of IL-7, <t>Flt3L,</t> thrombopoietin (ThPO) or SCF measured in sera of individual WT, NOD1 −/− and ΔCARD NOD1 ( n = 13) mice by <t>ELISA.</t> d , Total numbers of LT-HSCs (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 neg ), short-term HSCs (ST-HSCs) (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 + ), CLPs (Lin neg IL-7Rα + Flt3 + Sca1 inter c-kit inter ) and common myeloid progenitors (CMPs) (Lin neg IL-7Rα neg Sca-1 neg c-kit + CD16/32 neg CD34 + ) in the BM of WT, NOD1 −/− or ΔCARD NOD1 ( n = 16) mice. e , Frequency of donor cells in BM, thymus and spleen 8 weeks after reconstitution of irradiated H-2K/D d/b WT recipient mice ( n = 5) with a combination of equal numbers of congenically marked sort-purified HSCs (Lin neg IL-7Rα neg c-kit + Sca-1 + Flt3 neg ) from H-2K/D b/b WT (CD45.1/2 + ), NOD1 −/− (CD45.2 + ) and ΔCARD NOD1 (CD45.1 + ) animals as indicated on the diagram. a – d , Each symbol represents the value for an individual mouse. The bars indicate mean ± s.e.m. value for each group pooled from three experiments ( a – c ) and from one representative out of two independent experiments performed ( d ). Significance was determined by two-sided Mann–Whitney test in a , b and e and one-way ANOVA (Tukey’s multiple comparisons) test in c and d and are indicated by asterisks: * P < 0.05, ** P < 0.01, *** P < 0.001.
Stem Cell Factor, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio fms related tyrosine kinase 3 ligand flt3 l
a , Total cell numbers in BM, thymus, spleen and lymph nodes from WT ( n = 12), NOD1 −/− ( n = 10), ΔCARD NOD1 ( n = 10), NOD2 −/− ( n = 6) or MyD88 −/− ( n = 6) mice. b , Absolute numbers of T and B lymphocytes and NK cells in spleen from WT, NOD1 −/− or ΔCARD NOD1 ( n = 10) mice. c , Level of IL-7, <t>Flt3L,</t> thrombopoietin (ThPO) or SCF measured in sera of individual WT, NOD1 −/− and ΔCARD NOD1 ( n = 13) mice by <t>ELISA.</t> d , Total numbers of LT-HSCs (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 neg ), short-term HSCs (ST-HSCs) (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 + ), CLPs (Lin neg IL-7Rα + Flt3 + Sca1 inter c-kit inter ) and common myeloid progenitors (CMPs) (Lin neg IL-7Rα neg Sca-1 neg c-kit + CD16/32 neg CD34 + ) in the BM of WT, NOD1 −/− or ΔCARD NOD1 ( n = 16) mice. e , Frequency of donor cells in BM, thymus and spleen 8 weeks after reconstitution of irradiated H-2K/D d/b WT recipient mice ( n = 5) with a combination of equal numbers of congenically marked sort-purified HSCs (Lin neg IL-7Rα neg c-kit + Sca-1 + Flt3 neg ) from H-2K/D b/b WT (CD45.1/2 + ), NOD1 −/− (CD45.2 + ) and ΔCARD NOD1 (CD45.1 + ) animals as indicated on the diagram. a – d , Each symbol represents the value for an individual mouse. The bars indicate mean ± s.e.m. value for each group pooled from three experiments ( a – c ) and from one representative out of two independent experiments performed ( d ). Significance was determined by two-sided Mann–Whitney test in a , b and e and one-way ANOVA (Tukey’s multiple comparisons) test in c and d and are indicated by asterisks: * P < 0.05, ** P < 0.01, *** P < 0.001.
Fms Related Tyrosine Kinase 3 Ligand Flt3 L, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio mouse flt 3 ligand picokine elisa kit
a , Total cell numbers in BM, thymus, spleen and lymph nodes from WT ( n = 12), NOD1 −/− ( n = 10), ΔCARD NOD1 ( n = 10), NOD2 −/− ( n = 6) or MyD88 −/− ( n = 6) mice. b , Absolute numbers of T and B lymphocytes and NK cells in spleen from WT, NOD1 −/− or ΔCARD NOD1 ( n = 10) mice. c , Level of IL-7, <t>Flt3L,</t> thrombopoietin (ThPO) or SCF measured in sera of individual WT, NOD1 −/− and ΔCARD NOD1 ( n = 13) mice by <t>ELISA.</t> d , Total numbers of LT-HSCs (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 neg ), short-term HSCs (ST-HSCs) (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 + ), CLPs (Lin neg IL-7Rα + Flt3 + Sca1 inter c-kit inter ) and common myeloid progenitors (CMPs) (Lin neg IL-7Rα neg Sca-1 neg c-kit + CD16/32 neg CD34 + ) in the BM of WT, NOD1 −/− or ΔCARD NOD1 ( n = 16) mice. e , Frequency of donor cells in BM, thymus and spleen 8 weeks after reconstitution of irradiated H-2K/D d/b WT recipient mice ( n = 5) with a combination of equal numbers of congenically marked sort-purified HSCs (Lin neg IL-7Rα neg c-kit + Sca-1 + Flt3 neg ) from H-2K/D b/b WT (CD45.1/2 + ), NOD1 −/− (CD45.2 + ) and ΔCARD NOD1 (CD45.1 + ) animals as indicated on the diagram. a – d , Each symbol represents the value for an individual mouse. The bars indicate mean ± s.e.m. value for each group pooled from three experiments ( a – c ) and from one representative out of two independent experiments performed ( d ). Significance was determined by two-sided Mann–Whitney test in a , b and e and one-way ANOVA (Tukey’s multiple comparisons) test in c and d and are indicated by asterisks: * P < 0.05, ** P < 0.01, *** P < 0.001.
Mouse Flt 3 Ligand Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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An ELISA kit for the detection of Flt3L Mouse This uses Sandwich ELISA Double Antibody and has a sensitivity of 9 375pg ml
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a , Total cell numbers in BM, thymus, spleen and lymph nodes from WT ( n = 12), NOD1 −/− ( n = 10), ΔCARD NOD1 ( n = 10), NOD2 −/− ( n = 6) or MyD88 −/− ( n = 6) mice. b , Absolute numbers of T and B lymphocytes and NK cells in spleen from WT, NOD1 −/− or ΔCARD NOD1 ( n = 10) mice. c , Level of IL-7, Flt3L, thrombopoietin (ThPO) or SCF measured in sera of individual WT, NOD1 −/− and ΔCARD NOD1 ( n = 13) mice by ELISA. d , Total numbers of LT-HSCs (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 neg ), short-term HSCs (ST-HSCs) (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 + ), CLPs (Lin neg IL-7Rα + Flt3 + Sca1 inter c-kit inter ) and common myeloid progenitors (CMPs) (Lin neg IL-7Rα neg Sca-1 neg c-kit + CD16/32 neg CD34 + ) in the BM of WT, NOD1 −/− or ΔCARD NOD1 ( n = 16) mice. e , Frequency of donor cells in BM, thymus and spleen 8 weeks after reconstitution of irradiated H-2K/D d/b WT recipient mice ( n = 5) with a combination of equal numbers of congenically marked sort-purified HSCs (Lin neg IL-7Rα neg c-kit + Sca-1 + Flt3 neg ) from H-2K/D b/b WT (CD45.1/2 + ), NOD1 −/− (CD45.2 + ) and ΔCARD NOD1 (CD45.1 + ) animals as indicated on the diagram. a – d , Each symbol represents the value for an individual mouse. The bars indicate mean ± s.e.m. value for each group pooled from three experiments ( a – c ) and from one representative out of two independent experiments performed ( d ). Significance was determined by two-sided Mann–Whitney test in a , b and e and one-way ANOVA (Tukey’s multiple comparisons) test in c and d and are indicated by asterisks: * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Nature immunology

Article Title: Microbial ligand-independent regulation of lymphopoiesis by NOD1

doi: 10.1038/s41590-023-01668-x

Figure Lengend Snippet: a , Total cell numbers in BM, thymus, spleen and lymph nodes from WT ( n = 12), NOD1 −/− ( n = 10), ΔCARD NOD1 ( n = 10), NOD2 −/− ( n = 6) or MyD88 −/− ( n = 6) mice. b , Absolute numbers of T and B lymphocytes and NK cells in spleen from WT, NOD1 −/− or ΔCARD NOD1 ( n = 10) mice. c , Level of IL-7, Flt3L, thrombopoietin (ThPO) or SCF measured in sera of individual WT, NOD1 −/− and ΔCARD NOD1 ( n = 13) mice by ELISA. d , Total numbers of LT-HSCs (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 neg ), short-term HSCs (ST-HSCs) (Lin neg IL-7Rα neg Sca-1 + c-kit + Flt3 neg CD34 + ), CLPs (Lin neg IL-7Rα + Flt3 + Sca1 inter c-kit inter ) and common myeloid progenitors (CMPs) (Lin neg IL-7Rα neg Sca-1 neg c-kit + CD16/32 neg CD34 + ) in the BM of WT, NOD1 −/− or ΔCARD NOD1 ( n = 16) mice. e , Frequency of donor cells in BM, thymus and spleen 8 weeks after reconstitution of irradiated H-2K/D d/b WT recipient mice ( n = 5) with a combination of equal numbers of congenically marked sort-purified HSCs (Lin neg IL-7Rα neg c-kit + Sca-1 + Flt3 neg ) from H-2K/D b/b WT (CD45.1/2 + ), NOD1 −/− (CD45.2 + ) and ΔCARD NOD1 (CD45.1 + ) animals as indicated on the diagram. a – d , Each symbol represents the value for an individual mouse. The bars indicate mean ± s.e.m. value for each group pooled from three experiments ( a – c ) and from one representative out of two independent experiments performed ( d ). Significance was determined by two-sided Mann–Whitney test in a , b and e and one-way ANOVA (Tukey’s multiple comparisons) test in c and d and are indicated by asterisks: * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The hematopoietic cytokines were measured in sera of individual mice using Quantikine ELISA kits (R&D Systems: no. m700 for IL-7, no. MFK00 for Flt3L, no. MTP00 for thrombopoietin and no. MCK00 for SCF).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Irradiation, Purification, MANN-WHITNEY